Restriction Enzymes
New England Biolabs, Inc. BsmI – 2500 units
A restriction endonuclease that recognizes the sequence GAATG_CN^.
New England Biolabs, Inc. BsiWI-HF – 1500 units
A high fidelity restriction endonuclease that recognizes the sequence C^GTACG. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BsrDI – 200 units
A restriction endonuclease that recognizes the sequence GCAATG_NN^.
New England Biolabs, Inc. EarI – 500 units
A restriction endonuclease that recognizes the sequence CTCTTCN^NNN_.
New England Biolabs, Inc. BsaHI – 2000 units
A restriction endonuclease that recognizes the sequence GR^CG_YC.
New England Biolabs, Inc. AsiSI – 500 units
A restriction endonuclease that recognizes the sequence GCG_AT^CGC.
New England Biolabs, Inc. SwaI – 2000 units
A restriction endonuclease that recognizes the sequence ATTT_^AAAT.
New England Biolabs, Inc. AflII – 10000 units
A restriction endonuclease that recognizes the sequence C^TTAA_G.
New England Biolabs, Inc. BbsI – 300 units
A restriction endonuclease that recognizes the sequence GAAGACNN^NNNN_.
New England Biolabs, Inc. MscI – 1250 units
A restriction endonuclease that recognizes the sequence TGG_^CCA.
New England Biolabs, Inc. BceAI – 50 units
A restriction endonuclease that recognizes the sequence ACGGCNNNNNNNNNNNN^NN_.
New England Biolabs, Inc. BseYI – 100 units
A restriction endonuclease that recognizes the sequence C^CCAG_C.
New England Biolabs, Inc. I-SceI – 2500 units
A restriction endonuclease that recognizes the sequence TAGGG_ATAA^CAGGGTAAT. I-SceI is an intron-encoded endonuclease. The intron encoding I-SceI is present in the mitochondria of Saccharomyces cerevisiae.
New England Biolabs, Inc. I-CeuI – 500 units
A restriction endonuclease that recognizes the sequence CGTAACTATAACGGTC_CTAA^GGTAGCGAA.The intron encoding I-CeuI is present in the chloroplast large rRNA gene of Chlamydomonas eugametos (1). This gene has been cloned and overexpressed in E. coli (2).